IS1542 is an insertion sequence-like element which was originally identified in the orf2-vanR intergenic region of VanA resistance elements of glycopeptide-resistant Enterococcus faecium from hospital patients and from non-human sources in the UK. The nucleotide sequence of IS1542 was determined. It showed 81% homology with IS256 and contained an open reading frame sufficient to encode a 390 amino acid peptide predicted to have 87.2% identity with the transposase of IS256. By PCR, IS1542 was detected in the genomes of 26 of 28 (93%) VanA enterococci isolated from poultry in the UK and Ireland, but in only two of 65 (3%) glycopeptide-sensitive or VanC enterococci from hospital patients in the UK and Brazil. IS1542 may be a useful marker for evolutionary and epidemiological studies of VanA glycopeptide resistance in enterococci. Copyright (C) 1999 Federation of European Microbiological Societies.
CITATION STYLE
Da Costa Darini, A. L., Palepou, M. F. I., & Woodford, N. (1999). Nucleotide sequence of IS1542, an insertion sequence identified within VanA glycopeptide resistance elements of enterococci. FEMS Microbiology Letters, 173(2), 341–346. https://doi.org/10.1016/S0378-1097(99)00093-2
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