Optimal assembly strategies of transcriptome related to ploidies of eukaryotic organisms

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© 2015 He et al.; licensee Biomed Central.Background: Several transcriptome assemblers have been developed recently to assemble the short reads generated from the next-generation sequencing platforms and different strategies were employed for assembling transcriptomes of various eukaryotes without genome sequences. Though there are some comparisons among these assembly tools for assembling transcriptomes of different eukaryotic organisms, there is no report about the relationship between assembly strategies and ploidies of the organisms. Results: When we assembled transcriptomes of sweet potato (hexaploid), (a diploid fungus), (a diploid wild rice), five assemblers, including Edena, Oases, Soaptrans, IDBA-tran and Trinity, were used in different strategies (Single-Assembler Single-Parameter, SASP; Single-Assembler Multiple-Parameters, SAMP; Combined Transcriptome Assembly, CDTA, that is multiple assembler multiple parameter). It was found that CDTA strategy has the best performance compared with other two strategies for assembling transcriptome of the hexaploid sweet potato, whereas SAMP strategy with assembler Oases is better than other strategies for assembling transcriptomes of diploid fungus and the wild rice transcriptomes. Conclusion: Based on the results from ours and others, it is suggested that CDTA strategy is better used for transcriptome assembly of polyploidy organisms and SAMP strategy of Oases is outperformed for those diploid organisms without genome sequences.




He, B., Zhao, S., Chen, Y., Cao, Q., Wei, C., Cheng, X., & Zhang, Y. (2015). Optimal assembly strategies of transcriptome related to ploidies of eukaryotic organisms. BMC Genomics, 16(1). https://doi.org/10.1186/s12864-014-1192-7

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