Optimization of biohydrogen production from sugarcane bagasse by mixed cultures using a statistical method

10Citations
Citations of this article
46Readers
Mendeley users who have this article in their library.

Abstract

The aim of this study is to determine the optimum condition for biohydrogen production from sugarcane bagasse (SCB) hydrolysate using a central composite design and response surface methodology (RSM). SCB was hydrolyzed with 0.5% (v/v) sulfuric acid at 121 °C, 0.15 MPa for 60 min in an autoclave at a solid to liquid ratio of 1:15 (g:mL). Heat-treated bacterium obtained from a hydrogen producing fermentor was used as the inoculum. The interaction of three factors, i.e., substrate concentration, substrate:buffer ratio and inoculum:substrate ratio on hydrogen production potential (P) were investigated. The results indicated that the substrate concentration, substrate:buffer ratio and inoculum:substrate ratio had a significant influence effect on P. An optimal condition was found at substrate concentration of 22.77 g-total sugar L−1, 4.31 substrate:buffer ratio, and 0.31 inoculum:substrate ratio resulted in a maximum P of 6980 mL H2 L−1. The confirmation experiment results indicated that optimum P was statistically significant, from the predicted value obtained by RSM which suggests that RSM could be efficiently used to optimize a biohydrogen production from SCB hydrolysate using mixed cultures. These results indicates that the SCB hemicellulose hydrolysate is suitable as a fermentation media for producing biohydrogen. This approach will add value to SCB by converting agricultural waste into a safe and clean form of energy.

Cite

CITATION STYLE

APA

Sangyoka, S., Reungsang, A., & Lin, C. Y. (2016). Optimization of biohydrogen production from sugarcane bagasse by mixed cultures using a statistical method. Sustainable Environment Research, 26(5), 235–242. https://doi.org/10.1016/j.serj.2016.05.001

Register to see more suggestions

Mendeley helps you to discover research relevant for your work.

Already have an account?

Save time finding and organizing research with Mendeley

Sign up for free