Cordycepin is one of the most important bioactive compounds produced by species of Cordyceps sensu lato , but it is hard to produce large amounts of this substance in industrial production. In this work, single factor design, Plackett-Burman design, and central composite design were employed to establish the key factors and identify optimal culture conditions which improved cordycepin production. Using these culture conditions, a maximum production of cordycepin was 2008.48 mg/L for 700 mL working volume in the 1000 mL glass jars and total content of cordycepin reached 1405.94 mg/bottle. This method provides an effective way for increasing the cordycepin production at a large scale. The strategies used in this study could have a wide application in other fermentation processes.
Kang, C., Wen, T. C., Kang, J. C., Meng, Z. B., Li, G. R., & Hyde, K. D. (2014). Optimization of large-scale culture conditions for the production of cordycepin with cordyceps militaris by liquid static culture. Scientific World Journal, 2014. https://doi.org/10.1155/2014/510627