Osteogenic differentiation of adipose-derived mesenchymal stem cells into Polycaprolactone (PCL) scaffold

9Citations
Citations of this article
31Readers
Mendeley users who have this article in their library.

Abstract

The objective of this study was to evaluate the osteogenic differentiation of adipose-derived mesenchymal stem cells (ADSCs) into polycaprolactone scaffold (PCL). After ADSC characterization, cells were seeded onto the scaffolds and incubated with basal or osteogenic differentiating medium to MTT assay and osteogenesis evaluation. MTT cell viability assay showed that cell-seeded scaffolds cultured in both mediums presented formazan cristals formed as a result of the presence of viable cells. The osteogenic assay showed that cell-seeded PCL scaffolds cultured in osteogenic medium presented significantly greater amount of staining dye when compared to basal medium and scaffold without cells. The PCL scaffold have great potential to be used associated to ADSCs in Tissue Engineering to bone tissue regeneration.

Cite

CITATION STYLE

APA

Caetano, G. F., Bártolo, P. J., Domingos, M., Oliveira, C. C., Leite, M. N., & Frade, M. A. C. (2015). Osteogenic differentiation of adipose-derived mesenchymal stem cells into Polycaprolactone (PCL) scaffold. In Procedia Engineering (Vol. 110, pp. 59–66). Elsevier Ltd. https://doi.org/10.1016/j.proeng.2015.07.010

Register to see more suggestions

Mendeley helps you to discover research relevant for your work.

Already have an account?

Save time finding and organizing research with Mendeley

Sign up for free