Overlapping T-cell epitopes in the group I allergen of Dermatophagoides species restricted by HLA-DP and HLA-DR class II molecules

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Abstract

The induction of IgE antibodies reactive with the group I allergen of Dermatophagoides species (house dust mite [HDM]), which comprise a major component of the allergic immune response in HDM-atopic individuals, is dependent on the functional activity of specific CD4 + T cells. In this report we demonstrate that for a particular HDM-atopic individual the T-cell response to the group I allergen of Dermatophagoides pteronyssinus (Der p I) is limited to a single region (residues 101-143) of the protein. By mapping the fine antigen specificity with T-cell clones, we observed that the sequence 101-131 of Der p I contains a cluster of at least three overlapping T-cell epitopes. Analysis of the HLA class II restriction specificity of the T-cell clones revealed that the T-cell epitope, residues 110-131, was restricted by HLA-DRB1 *0101. In contrast, peptide Der p 1, 110-119 was recognized in association with HLA-DPB1 *0402. However, the ability of cloned T cells to proliferate to the peptide Der p 1, 107-119 presented by HLA-DPB1 *0401, HLA-DPB1 *0402, and HLA-DPB1 *0501 expressing accessory cells illustrates the heterogeneity of the restriction specificity of this region of Der p I. The application of this information in the design of peptide-based immunotherapy in the management of allergic responses to HDM is discussed. © 1994.

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Higgins, J. A., Thorpe, C. J., Hayball, J. D., O’Hehir, R. E., & Lamb, J. R. (1994). Overlapping T-cell epitopes in the group I allergen of Dermatophagoides species restricted by HLA-DP and HLA-DR class II molecules. The Journal of Allergy and Clinical Immunology, 93(5), 891–899. https://doi.org/10.1016/0091-6749(94)90383-2

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