The parallel reaction monitoring (PRM) assay has emerged as an alternative method of targeted quantification. The PRM assay is performed in a high resolution and high mass accuracy mode on a mass spectrometer. This review presents the features that make PRM a highly specific and selective method for targeted quantification using quadrupole-Orbitrap hybrid instruments. In addition, this review discusses the label-based and label-free methods of quantification that can be performed with the targeted approach.
Rauniyar, N. (2015, December 2). Parallel reaction monitoring: A targeted experiment performed using high resolution and high mass accuracy mass spectrometry. International Journal of Molecular Sciences. MDPI AG. https://doi.org/10.3390/ijms161226120