Use of PB-Cre4 Mice for Mosaic Gene Deletion

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Abstract

Transgene expression from short promoters in transgenic animals can lead to unwanted transgene expression patterns, often as a byproduct of random integration of the expression cassette into the host genome. Here I demonstrate that the often used {PB-Cre4} line (also referred to as {"Probasin-Cre")}, although expressing exclusively in the male prostate epithelium when transmitted through male mice, can lead to recombination of {loxP-flanked} alleles in a large variety of tissues when transmitted through female mice. This aberrant Cre activity due to Cre expression in the oocytes leads to different outcomes for maternally or paternally transmitted {loxP-flanked} alleles: Maternally inherited {loxP-flanked} alleles undergo recombination very efficiently, making female {PB-Cre4} mice an efficient monoallelic {"Cre} deleter line". However, paternally inherited {loxP-flanked} alleles are inefficiently recombined by maternal {PB-Cre4}, giving rise to mosaic expression patterns in the offspring. This mosaic recombination is difficult to detect with standard genotyping approaches of many mouse lines and should therefore caution researchers using {PB-Cre4} to use additional approaches to exclude the presence of recombined alleles. However, mosaic recombination should also be useful in transgenic "knockout" approaches for mosaic gene deletion experiments.

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APA

Birbach, A. (2013). Use of PB-Cre4 Mice for Mosaic Gene Deletion. PLoS ONE, 8(1). https://doi.org/10.1371/journal.pone.0053501

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