The PEF family proteins sorcin and grancalcin interact in vivo and in vitro

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The penta-EF hand (PEF) family of calcium binding proteins includes grancalcin, peflin, sorcin, calpain large and small subunits as well as ALG-2. Systematic testing of the heterodimerization abilities of the PEF proteins using the yeast two-hybrid and glutathione S-transferase pull-down assays revealed the new finding that grancalcin interacts strongly with sorcin. In addition, sorcin and grancalcin can be co-immunoprecipitated from lysates of human umbilical vein endothelial cells. Our results indicate that heterodimerization, in addition to differential interactions with target proteins, might be a way to regulate and fine tune processes mediated by calcium binding proteins of the penta-EF hand type. © 2003 Federation of European Biochemical Societies. Published by Elsevier Science B.V. All rights reserved.




Hansen, C., Tarabykina, S., La Cour, J. M., Lollike, K., & Berchtold, M. W. (2003). The PEF family proteins sorcin and grancalcin interact in vivo and in vitro. FEBS Letters, 545(2–3), 151–154.

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