Permissive role of reduced inwardly-rectifying potassium current density in the automaticity of the guinea pig pulmonary vein myocardium

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Abstract

The electrophysiological properties underlying the automaticity of the guinea pig pulmonary vein myocardium were studied. About 30% of the isolated pulmonary vein tissue preparations showed spontaneous electrical activity, as shown by glass microelectrode recordings from their myocardial layer. The remaining quiescent preparations had a resting membrane potential less negative than that in the left atria. Blockade of the acetylcholine activated potassium current (I K-ACh ) by tertiapin induced a depolarizing shift of the resting membrane potential and automatic electrical activity in the pulmonary vein, but not in the atria. The tertiapin-induced electrical activity, as well as the spontaneous activity, was inhibited by the application of carbachol or by chelation of intracellular Ca 2+ by BAPTA. The isolated pulmonary vein cardiomyocytes had an I K-ACh density similar to that of the atrial cardiomyocytes, but a lower density of the inwardly-rectifying potassium current (I K1 ). Spontaneous Ca 2+ transients were observed in about 30% of the isolated pulmonary vein cardiomyocytes, but not in atrial cardiomyocytes. The Ca 2+ transients in the pulmonary vein cardiomyocytes were induced by tertiapin and inhibited by carbachol. These results indicate that the pulmonary vein cardiomyocytes have a reduced density of the inwardly-rectifying potassium current, which plays a permissive role in their intracellular Ca 2+ -dependent automaticity.

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Tsuneoka, Y., Irie, M., Tanaka, Y., Sugimoto, T., Kobayashi, Y., Kusakabe, T., … Tanaka, H. (2017). Permissive role of reduced inwardly-rectifying potassium current density in the automaticity of the guinea pig pulmonary vein myocardium. Journal of Pharmacological Sciences, 133(4), 195–202. https://doi.org/10.1016/j.jphs.2016.12.006

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