PE-PGRS proteins localize in the mycobacterial cell wall and the cell wall localization of PE-PGRS33 has been shown to be attributed to its PE domain. In this study, we expressed deletion mutants of PE-PGRS30 in Mycobacterium smegmatis to characterize the role of its domains in protein localization. It was revealed that, apart from the PE domain, the C-terminal domain present in few PE-PGRS proteins carries individual cell wall localization signals. Proteinase K sensitivity assay showed that PE-PGRS30 is exposed on the mycobacterial surface through its PGRS domain. PGRS domain was also shown to be responsible for polar localization of PE-PGRS30. © 2014 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.
Chatrath, S., Gupta, V. K., & Garg, L. C. (2014). The PGRS domain is responsible for translocation of PE-PGRS30 to cell poles while the PE and the C-terminal domains localize it to the cell wall. FEBS Letters, 588(6), 990–994. https://doi.org/10.1016/j.febslet.2014.01.059