Monte Carlo simulations of fluorescence recovery after photobleaching (FRAP) experiments on two-component lipid bilayers systems in the solid- fluid phase coexistence region were carried out to study the geometry and size of fluid domains in these bilayers. The gel phase was simulated by superposable elliptical domains, which were either of predetermined dimensions, increasing in number with increasing gel phase fraction, or of predetermined number, increasing in dimensions with increasing gel phase fraction. The simulations were done from two perspectives: 1) a time- independent analysis of fractional fluorescence recovery as a function of fractional fluid phase in the system; and 2) a time-dependent analysis of fractional fluorescence recovery as a function of time at a given fraction of fluid phase in the system. The time-dependent simulations result in recovery curves that are directly comparable to experimental FRAP curves and provide topological and geometrical models for the coexisting phases that are consistent with the experimental result.
Coelho, F. P., Vaz, W. L. C., & Melo, E. (1997). Phase topology and percolation in two-component lipid bilayers: A Monte Carlo approach. Biophysical Journal, 72(4), 1501–1511. https://doi.org/10.1016/S0006-3495(97)78798-8