Phospholipase D1 (PLD1) is an important enzyme involved in lipid signal transduction in eukaryotes. A role for PLD1 in signaling in Saccharomyces cerevisiae was examined. Pheromone response in yeast is controlled by a well-characterized protein kinase cascade. Loss of PLD1 activity was found to impair pheromone-induced changes in cellular morphology that result in formation of mating projections. The rate at which projections appeared following pheromone treatment was delayed, suggesting that PLD1 facilitates the execution of a rate-limiting step in morphogenesis. Mutants were found to be less sensitive to pheromone, again arguing that PLD1 is acting at a rate-limiting step. The fact that morphogenesis is most dramatically affected indicates that PLD1 functions primarily in the morphogenic branch of the pheromone response pathway. © 2001 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved.
Hairfield, M. L., Ayers, A. B., & Dolan, J. W. (2001). Phospholipase D1 is required for efficient mating projection formation in Saccharomyces cerevisiae. FEMS Yeast Research, 1(3), 225–232. https://doi.org/10.1016/S1567-1356(01)00027-7