Phosphopeptide binding by Sld3 links Dbf4‐dependent kinase to MCM replicative helicase activation

  • Deegan T
  • Yeeles J
  • Diffley J
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Abstract

The initiation of eukaryotic DNA replication requires the assembly of active CMG (Cdc45-MCM-GINS) helicases at replication origins by a set of conserved and essential firing factors. This process is controlled during the cell cycle by cyclin-dependent kinase (CDK) and Dbf4-dependent kinase (DDK), and in response to DNA damage by the checkpoint kinase Rad53/Chk1. Here we show that Sld3, previously shown to be an essential CDK and Rad53 substrate, is recruited to the inactive MCM double hexamer in a DDK-dependent manner. Sld3 binds specifically to DDK-phosphorylated peptides from two MCM subunits (Mcm4, 6) and then recruits Cdc45. MCM mutants that cannot bind Sld3 or Sld3 mutants that cannot bind phospho-MCM or Cdc45 do not support replication. Moreover, phosphomimicking mutants in Mcm4 and Mcm6 bind Sld3 without DDK and facilitate DDK-independent replication. Thus, Sld3 is an essential "reader" of DDK phosphorylation, integrating signals from three distinct protein kinase pathways to coordinate DNA replication during S phase.

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Deegan, T. D., Yeeles, J. T., & Diffley, J. F. (2016). Phosphopeptide binding by Sld3 links Dbf4‐dependent kinase to MCM replicative helicase activation . The EMBO Journal, 35(9), 961–973. https://doi.org/10.15252/embj.201593552

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