A phosphorylation tag for uranyl mediated protein purification and photo assisted tag removal

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Abstract

Most protein purification procedures include an affinity tag fused to either the N or C-terminal end of the protein of interest as well as a procedure for tag removal. Tag removal is not straightforward and especially tag removal from the C-terminal end is a challenge due to the characteristics of enzymes available for this purpose. In the present study, we demonstrate the utility of the divalent uranyl ion in a new procedure for protein purification and tag removal. By employment of a GFP (green florescence protein) recombinant protein we show that uranyl binding to a phosphorylated C-terminal tag enables target protein purification from an E. coli extract by immobilized uranyl affinity chromatography. Subsequently, the tag can be efficiently removed by UV-irradiation assisted uranyl photocleavage. We therefore suggest that the divalent uranyl ion (UO22+) may provide a dual function in protein purification and subsequent C-terminal tag removal procedures. © 2014 Zhang et al.

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APA

Zhang, Q., Jørgensen, T. J. D., Nielsen, P. E., & Møllegaard, N. E. (2014). A phosphorylation tag for uranyl mediated protein purification and photo assisted tag removal. PLoS ONE, 9(3). https://doi.org/10.1371/journal.pone.0091138

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