Phototransformation and proton pumping activity of the 14-fluoro bacteriorhodopsin derivatives

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Abstract

The photoinduced behavior and proton pumping characteristics of some bacteriorhodopsin (BR) analogs with fluorinated chromophores (all-trans 14-fluorinated [14-F] retinal and 13-cis 14-F retinal) derived from wild type (WT) and D96N mutant BR were investigated. These analogs were characterized using spectrophotometry and a highly sensitive electrochemical technique. Similar to the white membrane JW2N, the apomembranes WT ET 1000 and D96N form photoactive pigments with the 14-F chromophores. The resulting analogs have a major absorption band at 588 nm. Red-shifted pigment (λ(max)≤680 nm) has been previously observed as a minor component of the major 587-nm pigment in 14-F BR made with white membrane JW2N. A similar red-shifted pigment is formed under yellow light (λ>500 nm) only in the 14-F analogs derived from WT ET 1000. The measurements of the photoinduced transformation in 14-F WT analogs show that the photocycle of the major pigment occurs simultaneously with the process in the red region and is partially masked by the formation of the red-shifted species. The 14-F D96N samples have a significantly slower and more complicated photoinduced behavior. Electrochemical measurements show that the photoinduced transformation of the red species is not accompanied by proton transport. Copyright (C) 1998 Elsevier Science B.V.

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Druzhko, A. B., Robertson, B., Alvarez, R., De Lera, A. R., & Weetall, H. H. (1998). Phototransformation and proton pumping activity of the 14-fluoro bacteriorhodopsin derivatives. Biochimica et Biophysica Acta - Biomembranes, 1371(2), 371–381. https://doi.org/10.1016/S0005-2736(98)00038-8

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