Plant calcium-dependent protein kinase-related kinases (CRKs) do not require calcium for their activities

46Citations
Citations of this article
21Readers
Mendeley users who have this article in their library.

Abstract

In plants, calcium-dependent protein kinases (CDPKs) make up a large family that is characterized by a C-terminal calmodulin(CaM)-like domain. Recently, a novel carrot cDNA clone encoding an atypical CDPK, which has a significantly degenerate sequence in the CaM-like domain, was found and named CDPK-related protein kinase (CRK) [Lindzen, E. and Choi, J.H. (1995) Plant Mol. Biol. 28, 785-797]. We obtained two different cDNA clones from maize which encode CRKs. For the first enzymatic characterization of CRK, a maize cDNA clone was expressed in E. coli. The recombinant protein efficiently phosphorylated casein, a conventional protein substrate, Notably, in this in vitro phosphorylation assay, the kinase activity did not require calcium as an activator. Thus, CRKs were suggested to be novel calcium-independent protein kinases having a degenerate CaM domain, the function of which remains to be elucidated.

Cite

CITATION STYLE

APA

Furumoto, T., Ogawa, N., Hata, S., & Izui, K. (1996). Plant calcium-dependent protein kinase-related kinases (CRKs) do not require calcium for their activities. FEBS Letters, 396(2–3), 147–151. https://doi.org/10.1016/0014-5793(96)01090-3

Register to see more suggestions

Mendeley helps you to discover research relevant for your work.

Already have an account?

Save time finding and organizing research with Mendeley

Sign up for free