A chemiluminescence study showed that Qi site inhibitors such as antimycin A induce O2- generation in respiring cyanide-sensitive mitochondria from the yeast, Hansenula anomala. The O2- generation was suppressed by radical scavengers such as flavone, butylated hydroxyanisole, and Co0. Induction of cyanide-resistant respiration in H. anomala cells by Qi site inhibitors was also inhibited by these radical scavengers. Furthermore, antimycin A-induced synthesis of the mitochondrial 36-kDa protein, which is thought to be the alternative oxidase functional in the cyanide-resistant respiratory pathway, was abolished by the addition of flavone. These observations suggest that O2- is somehow involved in the induction of cyanide-resistant respiration. © 1992.
Minagawa, N., Koga, S., Nakano, M., Sakajo, S., & Yoshimoto, A. (1992). Possible involvement of superoxide anion in the induction of cyanide-resistant respiration in Hansenula anomala. FEBS Letters, 302(3), 217–219. https://doi.org/10.1016/0014-5793(92)80444-L