Postsynaptically silent synapses in single neuron cultures

110Citations
Citations of this article
121Readers
Mendeley users who have this article in their library.

Abstract

We have used the synapses that isolated hippocampal cells in culture form onto themselves (autapses) to determine if some synapses lack functional AMPA receptors (AMPARs). A comparison of the synaptic variability of the AMPAR- and NMDAR-mediated evoked responses, as well as of miniature synaptic responses, indicates that a population of events exists that only contains an NMDAR component. Spillover of glutamate from adjacent synapses cannot explain these results because in single cell cultures all synaptic events mediated by AMPARs should be detected. Immunocytochemical analysis of these cultures clearly reveals a population of synapses with puncta for NR1 (NMDAR) but not for GluR1 (AMPAR). These results provide strong anatomical and physiological evidence for the existence of postsynaptically silent synapses.

Cite

CITATION STYLE

APA

Gomperts, S. N., Rao, A., Craig, A. M., Malenka, R. C., & Nicoll, R. A. (1998). Postsynaptically silent synapses in single neuron cultures. Neuron, 21(6), 1443–1451. https://doi.org/10.1016/S0896-6273(00)80662-5

Register to see more suggestions

Mendeley helps you to discover research relevant for your work.

Already have an account?

Save time finding and organizing research with Mendeley

Sign up for free