Stroke is a multifactorial disease that may be associated with aberrant DNA methylation profiles. We investigated epigenetic dysregulation for the methylenetetrahydrofolate reductase ( MTHFR ) gene among ischemic stroke patients. Cases and controls were recruited after obtaining signed written informed consents following a screening process against the inclusion/exclusion criteria. Serum vitamin profiles (folate, vitamin B 12 , and homocysteine) were determined using immunoassays. Methylation profiles for CpGs A and B in the MTHFR gene were determined using a bisulfite-pyrosequencing method. Methylation of MTHFR significantly increased the susceptibility risk for ischemic stroke. In particular, CpG A outperformed CpG B in mediating serum folate and vitamin B 12 levels to increase ischemic stroke susceptibility risks by 4.73-fold. However, both CpGs A and B were not associated with serum homocysteine levels or ischemic stroke severity. CpG A is a potential epigenetic marker in mediating serum folate and vitamin B 12 to contribute to ischemic stroke.
Wei, L. K., Sutherland, H., Au, A., Camilleri, E., Haupt, L. M., Gan, S. H., & Griffiths, L. R. (2015). A potential epigenetic marker mediating serum folate and vitamin Blevels contributes to the risk of ischemic stroke. BioMed Research International, 2015. https://doi.org/10.1155/2015/167976