To investigate the functionality of A2B adenosine receptor ( A2B AR) and the nitric oxide (NO) and vascular endothelial growth factor (VEGF) signaling pathway in the endothelial cell proliferation/migration during preeclampsia, we used human umbilical vein endothelial cells (HUVECs) isolated from normal pregnancies n=15 or pregnancies with preeclampsia n=15 . Experiments were performed in presence or absence of the nonselective adenosine receptor agonist NECA, the A2B AR selective antagonist MRS-1754, and the nitric oxide synthase (NOS) inhibitor L-NAME. Results indicated that cells from preeclampsia exhibited a significant higher protein level of A2B AR and logEC 50 for NECA-mediated proliferation than normotensive pregnancies. The stimulatory effect of NECA (10 μ M, 24 h) on cell proliferation was prevented by MRS-1754 (5 nM) coincubation only in cells from normotensive pregnancies. Nevertheless, L-NAME (100 μ M, 24 h) reduced the NECA-induced cell proliferation/migration in HUVEC from normal pregnancy; however in preeclampsia only NECA-induced cell proliferation was reduced by L-NAME. Moreover, NECA increased protein nitration and abundance of VEGF in cells from normal pregnancy and effect prevented by MRS-1754 coincubation. Nevertheless, in preeclampsia NECA did not affect the protein level of VEGF. In conclusion HUVECs from preeclampsia exhibit elevated protein level of A2B AR and impairment of A2B AR-mediated NO/VEGF signaling pathway.
Acurio, J., Troncoso, F., Bertoglia, P., Salomon, C., Aguayo, C., Sobrevia, L., & Escudero, C. (2014). Potential role of A2B adenosine receptors on proliferation/migration of fetal endothelium derived from preeclamptic pregnancies. BioMed Research International, 2014. https://doi.org/10.1155/2014/274507