A PPARγ ligand present in Actinidia fruit (Actinidia chrysantha) is identified as dilinolenoyl galactosyl glycerol

1Citations
Citations of this article
6Readers
Mendeley users who have this article in their library.

Abstract

Activity-guided fractionation of Actinidia fruit species, including Kiwifruit, has identified DLGG (dilinolenoyl galactosyl glycerol) as a potent PPARγ (peroxisome-proliferator-activated receptor γ) ligand. DLGG is a type of MGDG (monogalactosyl diacylglycerol) and is present in all chloroplasts, and therefore all green fruits and vegetables. PPARγ is a ligand-activated transcription factor that regulates glucose metabolism and inflammation. An ethyl acetate extract of Actinidia chrysantha was fractionated by HPLC and the PPARγ-binding activity was detected by FP (fluorescence polarization). Linoleic and α-linolenic acids in A. chrysantha were readily detected as PPARγ ligands. Slow-binding PPARγ ligands were detected in several hydrophobic fractions. High-resolution MS identified DLGG as one of these ligands and confirmed that its binding is non-covalent. DLGG is a slow-binding PPARγ ligand with an IC 50 of 1.64 μM, 0.093 achieved after 45 min equilibration. DLGG is the first example of a form of DAG (diacylglycerol) that is a PPARγ ligand. In addition, DLGG is the first reported glycolipid ligand for PPARγ and also the first non-covalent, slow-binding PPARγ ligand. © 2013 The Author(s).

Cite

CITATION STYLE

APA

Martin, H., McGhie, T. K., & Lunken, R. C. M. (2013). A PPARγ ligand present in Actinidia fruit (Actinidia chrysantha) is identified as dilinolenoyl galactosyl glycerol. Bioscience Reports, 33(3), 387–394. https://doi.org/10.1042/BSR20120110

Register to see more suggestions

Mendeley helps you to discover research relevant for your work.

Already have an account?

Save time finding and organizing research with Mendeley

Sign up for free