Prevalence and molecular identification of Cryptosporidium isolates from pet lizards and snakes in Italy

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Abstract

In order to acquire prevalence and genetic data on Cryptosporidium infections in captive lizards and snakes kept as pets, a survey was conducted on 150 individual reptiles from southern Italy. Fecal samples were preserved in 5% formalin and analyzed using a commercial immunofluorescence assay (IFA) for the detection of Cryptosporidium oocysts and Giardia cysts. IFA revealed the presence of Cryptosporidium oocysts in nine of the 150 samples examined (6.0%), precisely in 6/125 snakes (4.8%) and in 3/25 lizards (12.0%); all fecal samples tested negative for the presence of Giardia cysts. Molecular characterization based on nested PCR amplification and sequencing of the SSU-rRNA gene, revealed the presence of Cryptosporidium serpentis in three samples from snakes (Boa constrictor constrictor, Elapheguttata guttata guttata and Python molurus).

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APA

Rinaldi, L., Capasso, M., Mihalca, A. D., Cirillo, R., Cringoli, G., & Cacciò, S. (2012). Prevalence and molecular identification of Cryptosporidium isolates from pet lizards and snakes in Italy. Parasite (Paris, France), 19(4), 437–440. https://doi.org/10.1051/parasite/2012194437

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