A strand-specific transcriptome sequencing strategy, directional ligation sequencing or DeLi-seq, was employed to profile antisense transcriptome of Schizosaccharomyces pombe. Under both normal and heat shock conditions, we found that polyadenylated antisense transcripts are broadly expressed while distinct expression patterns were observed for protein-coding and non-coding loci. Dominant antisense expression is enriched in protein-coding genes involved in meiosis or stress response pathways. Detailed analyses further suggest that antisense transcripts are independently regulated with respect to their sense transcripts, and diverse mechanisms might be potentially involved in the biogenesis and degradation of antisense RNAs. Taken together, antisense transcription may have profound impacts on global gene regulation in S. pombe.
T., N., K., T., Z., W., S., S., H., W., B., X., … Y., G. (2010). The prevalence and regulation of antisense transcripts in Schizosaccharomyces pombe. PLoS ONE. J. Zhu, Institute for Genome Sciences and Policy, Duke University Medical Center, Durham, NC, United States. E-mail: firstname.lastname@example.org: Public Library of Science (185 Berry Street, Suite 1300, San Francisco CA 94107, United States). Retrieved from http://www.plosone.org/article/fetchObjectAttachment.action?uri=info%3Adoi%2F10.1371%2Fjournal.pone.0015271&representation=PDF