Prion strain identification has been hitherto achieved using time-consuming incubation time determinations in one or more mouse lines and elaborate neuropathological assessment. In the present work, we make a detailed study of the properties of PrP-overproducing Tga20 mice. We show that in these mice the four prion strains examined are rapidly and faithfully amplified and can subsequently be discriminated by a cell-based procedure, the Cell Panel Assay.
Karapetyan, Y. E., Saá, P., Mahal, S. P., Sferrazza, G. F., Sherman, A., Salès, N., … Lasmézas, C. I. (2009). Prion strain discrimination based on rapid in vivo amplification and analysis by the cell panel assay. PLoS ONE, 4(5). https://doi.org/10.1371/journal.pone.0005730