Production of two different catalase-peroxidases by Deinococcus radiophilus

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Abstract

The production of two kinds of catalase-peroxidase, viz. catalase-2 and catalase-3 of Deinococcus radiophilus varied depending upon growth phases and oxidative stress. A gradual increase in total catalase activity occurred during exponential and stationary phase. Electrophoretic resolution of these catalases in Deinococcal cell extracts revealed the uniform occurrence of catalase-2 and the appearance of catalase-3 only during the late exponential and stationary phase. A substantial increase in total catalase was observed in either hydrogen peroxide- or UV-treated cells. Monitoring of D. radiophilus catalase activity in the oxidative stressed and non-treated cells by gel electrophoresis followed by densitometry revealed the several-fold increase in catalase-3, which is above the constant level of catalase-2. The occurrence of catalase-3 and catalase-2 revealed by fractionation of sucrose-shocked cells suggests that catalase-3 is a cytosolic inducible enzyme whereas catalase-2 is the membrane-associated constitutive enzyme. Copyright (C) 2000 Federation of European Microbiological Societies.

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Yun, E. J., & Lee, Y. N. (2000). Production of two different catalase-peroxidases by Deinococcus radiophilus. FEMS Microbiology Letters, 184(2), 155–159. https://doi.org/10.1016/S0378-1097(00)00035-5

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