Protective effect of diphenyl diselenide against peroxynitrite-mediated endothelial cell death: A comparison with ebselen

43Citations
Citations of this article
25Readers
Mendeley users who have this article in their library.

Abstract

Excess production of superoxide (O2-) and nitric oxide (NO) in blood vessel walls may occur early in atherogenesis leading to the formation of peroxynitrite, a strong oxidant and nitrating agent. This study was designed to determine the effect of diphenyl diselenide (PhSe)2, a synthetic organoselenium compound, in comparison with ebselen, on peroxynitrite-mediated endothelial damage. Experimental results showed that pre-incubation of BAEC (24 h) with low concentrations of (PhSe)2 (0.5 and 1 μM) protected the cells from peroxynitrite-dependent apoptosis and protein tyrosine nitration. The intracellular levels of GSH were almost completely depleted by peroxynitrite and, although the compounds did not restore its normal levels, (PhSe)2 per se significantly increased GSH in a concentration-dependent manner. Moreover, (PhSe)2, which was about two times more active as a GPx mimic than ebselen, induced a significantly higher increase in both cellular GPx expression and activity. Taking into account the kinetics of the reaction between peroxynitrite and (PhSe) 2, our data indicate that (PhSe)2 protects BAEC against peroxynitrite-mediated cell damage not by a direct reaction, but rather by increasing cellular GPx expression as a consequence of enhanced nuclear translocation of Nrf-2, which together with the increase in intracellular GSH, may work catalytically to reduce peroxynitrite to nitrite. © 2013 Published by Elsevier Inc.

Cite

CITATION STYLE

APA

De Bem, A. F., Fiuza, B., Calcerrada, P., Brito, P. M., Peluffo, G., Dinis, T. C. P., … Almeida, L. M. (2013). Protective effect of diphenyl diselenide against peroxynitrite-mediated endothelial cell death: A comparison with ebselen. Nitric Oxide - Biology and Chemistry, 31, 20–30. https://doi.org/10.1016/j.niox.2013.03.003

Register to see more suggestions

Mendeley helps you to discover research relevant for your work.

Already have an account?

Save time finding and organizing research with Mendeley

Sign up for free