Many protein-protein interactions are mediated through independently folding modular domains. Proteome-wide efforts to model protein-protein interaction or "interactome" networks have largely ignored this modular organization of proteins. We developed an experimental strategy to efficiently identify interaction domains and generated a domain-based interactome network for proteins involved in C. elegans early-embryonic cell divisions. Minimal interacting regions were identified for over 200 proteins, providing important information on their domain organization. Furthermore, our approach increased the sensitivity of the two-hybrid system, resulting in a more complete interactome network. This interactome modeling strategy revealed insights into C. elegans centrosome function and is applicable to other biological processes in this and other organisms. © 2008 Elsevier Inc. All rights reserved.
Boxem, M., Maliga, Z., Klitgord, N., Li, N., Lemmens, I., Mana, M., … Vidal, M. (2008). A Protein Domain-Based Interactome Network for C. elegans Early Embryogenesis. Cell, 134(3), 534–545. https://doi.org/10.1016/j.cell.2008.07.009