The proteome of mouse vestibular hair bundles over development

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Abstract

Development of the vertebrate hair bundle is a precisely orchestrated event that culminates in production of a tightly ordered arrangement of actin-rich stereocilia and a single axonemal kinocilium. To understand how the protein composition of the bundle changes during development, we isolated bundles from young (postnatal days P4-P6) and mature (P21-P25) mouse utricles using the twist-off method, then characterized their constituent proteins using liquid-chromatography tandem mass spectrometry with data-dependent acquisition. Using MaxQuant and label-free quantitation, we measured relative abundances of proteins in both bundles and in the whole utricle; comparison of protein abundance between the two fractions allows calculation of enrichment in bundles. These data, which are available via ProteomeXchange with identifier PXD002167, will be useful for examining the proteins present in mammalian vestibular bundles and how their concentrations change over development. Design Type(s) parallel group design • replicate design • organism development design Measurement Type(s) protein expression profiling Technology Type(s) mass spectrometry assay Factor Type(s) life cycle stage Sample Characteristic(s) Mus musculus • utricle of membranous labyrinth

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Krey, J. F., Sherman, N. E., Jeffery, E. D., Choi, D., & Barr-Gillespie, P. G. (2015). The proteome of mouse vestibular hair bundles over development. Scientific Data, 2. https://doi.org/10.1038/sdata.2015.47

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