Proteome-based analysis of serologically defined tumor-associated antigens in cutaneous lymphoma

13Citations
Citations of this article
15Readers
Mendeley users who have this article in their library.

Abstract

Information on specificities of serological responses against tumor cells in cutaneous lymphoma patients is relatively restricted. To advance the knowledge of serological immune responses against and to assess the scope of tumor antigenicity of cutaneous lymphoma, 1- and 2-dimensional Western blot analyses with sera from patients were combined with proteomics-based protein identification. Testing sera from 87 cutaneous lymphoma patients by 1-dimensional Western blot analysis, 64 cases of seroreactivity against lymphoma cells were found. The positive responses were relatively weak, restricted to few antigens in each case, and heterogeneous. To identify the antigens, proteins of the mycosis fungoides cell line MyLa and primary tumor cells were separated by 2-dimensional gel electrophoresis, Western-blotted and probed with heterogeneous and autologous patient sera. The antigens were identified from silver-stained replica gels by MALDI-TOF mass spectrometry. 14 different antigens were assigned and identified with this proteome-serological approach. Only one, vimentin, had been reported before, the other 13 are new antigens for cutaneous lymphomas.

Cite

CITATION STYLE

APA

Forgber, M., Gellrich, S., Sharav, T., Sterry, W., & Walden, P. (2009). Proteome-based analysis of serologically defined tumor-associated antigens in cutaneous lymphoma. PLoS ONE, 4(12). https://doi.org/10.1371/journal.pone.0008376

Register to see more suggestions

Mendeley helps you to discover research relevant for your work.

Already have an account?

Save time finding and organizing research with Mendeley

Sign up for free