Quantification of global transcription patterns in prokaryotes using spotted microarrays

31Citations
Citations of this article
44Readers
Mendeley users who have this article in their library.

Abstract

We describe an analysis, applicable to any spotted microarray dataset produced using genomic DNA as a reference, that quantifies prokaryotic levels of mRNA on a genome-wide scale. Applying this to Mycobacterium tuberculosis, we validate the technique, show a correlation between level of expression and biological importance, define the complement of invariant genes and analyze absolute levels of expression by functional class to develop ways of understanding an organism's biology without comparison to another growth condition. © 2007 Sidders et al.; licensee BioMed Central Ltd.

Cite

CITATION STYLE

APA

Sidders, B., Withers, M., Kendall, S. L., Bacon, J., Waddell, S. J., Hinds, J., … Stoker, N. G. (2007). Quantification of global transcription patterns in prokaryotes using spotted microarrays. Genome Biology, 8(12). https://doi.org/10.1186/gb-2007-8-12-r265

Register to see more suggestions

Mendeley helps you to discover research relevant for your work.

Already have an account?

Save time finding and organizing research with Mendeley

Sign up for free