Quantitative detection of rare interphase chromosome breaks and translocations by high-throughput imaging

9Citations
Citations of this article
17Readers
Mendeley users who have this article in their library.

Abstract

We report a method for the sensitive detection of rare chromosome breaks and translocations in interphase cells. HiBA-FISH (High-throughput break-apart FISH) combines high-throughput imaging with the measurement of the spatial separation of FISH probes flanking target genome regions of interest. As proof-of-principle, we apply hiBA-FISH to detect with high sensitivity and specificity rare chromosome breaks and translocations in the anaplastic large cell lymphoma breakpoint regions of NPM1 and ALK. This method complements existing approaches to detect translocations by overcoming the need for precise knowledge of translocation breakpoints and it extends traditional FISH by its quantitative nature.

Cite

CITATION STYLE

APA

Burman, B., Misteli, T., & Pegoraro, G. (2015). Quantitative detection of rare interphase chromosome breaks and translocations by high-throughput imaging. Genome Biology, 16(1). https://doi.org/10.1186/s13059-015-0718-x

Register to see more suggestions

Mendeley helps you to discover research relevant for your work.

Already have an account?

Save time finding and organizing research with Mendeley

Sign up for free