To investigate the contribution of ras-related signalling molecules to the mechanotransduction process, stretch-sensing human periodontal ligament (PDL) fibroblasts were isolated and cultured in dishes with a flexible bottom. The cells were stimulated by stretching the bottom of the dishes and membrane fractions were prepared and analysed at the level of mapping small GTP-binding proteins by high resolution two-dimensional gel electrophoresis followed by renaturing transfer and an [α- 32P]GTP-overlay procedure. This analysis revealed that mechanically-stretched PDL fibroblasts exhibit complete down-regulation of rhoA and induction of rab6, rab17 and a putative member of the rab3 subfamily in a cell type-specific manner. © 1995.
Basdra, E. K., Papavassiliou, A. G., & Huber, L. A. (1995). Rab and rho GTPases are involved in specific response of periodontal ligament fibroblasts to mechanical stretching. BBA - Molecular Cell Research, 1268(2), 209–213. https://doi.org/10.1016/0167-4889(95)00090-F