Rapid biochemical synthesis of 11C-labeled single chain variable fragment antibody for immuno-PET by cell-free protein synthesis

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Abstract

Immuno-PET is a promising approach for improved cancer diagnosis, by taking advantage of the high specificity of antibodies. Here, we present a novel cell-free protein synthesis method for preparing a positron emitter labeled-antibody. Functional anti-human EGFRvIII single chain Fv, MR1-1, was successfully labeled with carbon-11 (half-life = 20.4 min) in 5 min (36% yield) by the direct incorporation of the clinical PET tracer, l-[11C] methionine. The product [11C]MR1-1 was easily and rapidly isolated with high radiochemical purity (>95%) from the reaction solution, by affinity purification. This method would be widely applicable to the preparation of radiolabeled antibodies for PET imaging. © 2012 Elsevier Ltd. All rights reserved.

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Matsuda, T., Furumoto, S., Higuchi, K., Yokoyama, J., Zhang, M. R., Yanai, K., … Kigawa, T. (2012). Rapid biochemical synthesis of 11C-labeled single chain variable fragment antibody for immuno-PET by cell-free protein synthesis. Bioorganic and Medicinal Chemistry, 20(22), 6579–6582. https://doi.org/10.1016/j.bmc.2012.09.038

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