A rapid, extensive, and transient transcriptional response to estrogen signaling in breast cancer cells

271Citations
Citations of this article
444Readers
Mendeley users who have this article in their library.

Abstract

We report the immediate effects of estrogen signaling on the transcriptome of breast cancer cells using global run-on and sequencing (GRO-seq). The data were analyzed using a new bioinformatic approach that allowed us to identify transcripts directly from the GRO-seq data. We found that estrogen signaling directly regulates a strikingly large fraction of the transcriptome in a rapid, robust, and unexpectedly transient manner. In addition to protein-coding genes, estrogen regulates the distribution and activity of all three RNA polymerases and virtually every class of noncoding RNA that has been described to date. We also identified a large number of previously undetected estrogen-regulated intergenic transcripts, many of which are found proximal to estrogen receptor binding sites. Collectively, our results provide the most comprehensive measurement of the primary and immediate estrogen effects to date and a resource for understanding rapid signal-dependent transcription in other systems. © 2011 Elsevier Inc.

Cite

CITATION STYLE

APA

Hah, N., Danko, C. G., Core, L., Waterfall, J. J., Siepel, A., Lis, J. T., & Kraus, W. L. (2011). A rapid, extensive, and transient transcriptional response to estrogen signaling in breast cancer cells. Cell, 145(4), 622–634. https://doi.org/10.1016/j.cell.2011.03.042

Register to see more suggestions

Mendeley helps you to discover research relevant for your work.

Already have an account?

Save time finding and organizing research with Mendeley

Sign up for free