Rapid18 F-FDG uptake in brain of awake, behaving rat and anesthetized chicken has implications for behavioral PET studies in species with high metabolisms

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Abstract

Brain-behavior studies using18 F-FDGPET aimto reveal brain regions that become active during behavior. In standard protocols,18 F-FDG is injected, the behavior is executed during 30–60min of tracer uptake, and then the animal is anesthetized and scanned. Hence, the uptake of18 F-FDG is not itself observed and could, in fact, be complete in very little time. This has implications for behavioral studies because uptake is assumed to reflect concurrent behavior. Here, we utilized a new, miniature PET scanner termed RatCAP to measure uptake simultaneously with behavior. We employed a novel injection protocol in which we administered18 F-FDG (i.v.) four times over two 2h to allow for repeated measurements and the correlation of changes in uptake and behavioral activity. Furthermore, using standard PET methods, we explored the effects of injection route on uptake time in chickens, a model for avians, for which PET studies are just beginning. We found that in the awake, behaving rat most of the18 F-FDG uptake occurred within minutes and overlapped to a large extent with18 F-FDG data taken from longer uptake periods. By contrast, behavior which occurred within minutes of the18 F-FDG infusion differed markedly from the behavior that occurred during later uptake periods. Accordingly, we found that changes in18 F-FDG uptake in the striatum, motor cortex and cerebellum relative to different reference regions significantly predicted changes in behavioral activity during the scan, if the time bins used for correlation were near the injection times of18 F-FDG. However, when morphine was also injected during the scan, which completely abolished behavioral activity for over 50min, a large proportion of the variance in behavioral activity was also explained by the uptake data fromthe entire scan. In anesthetized chickens, tracer uptake was complete in about 80min with s.c. injection, but 8min with i.v. injection. In conclusion, uptake time needs to be taken into account to more accurately correlate PET and behavioral data in mammals and avians. Additionally, RatCAPtogether with multiple, successive injections of18 F-FDGmay be useful to explore changes in uptake over time in relation to changes in behavior.

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Gold, M. E. L., Norell, M. A., Budassi, M., Vaska, P., & Schulz, D. (2018). Rapid18 F-FDG uptake in brain of awake, behaving rat and anesthetized chicken has implications for behavioral PET studies in species with high metabolisms. Frontiers in Behavioral Neuroscience, 12. https://doi.org/10.3389/fnbeh.2018.00115

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