Reactivation of metal-requiring apoenzymes by phytochelatin-metal complexes

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Abstract

The enzymatically inactive, metal-requiring apoforms of diamino oxidase and of carbonic anhydrase were reactivated by copper- and zinc-phytochelatin complexes, respectively. The level and the rate of reactivation effected by metal complexes consisting of poly(γ-glutamylcysteinyl)glycine as well as by the respective free metal ion were compared. An efficient transfer of zinc and copper from phytochelatin-complexes to apoenzymes was observed in vitro. © 1991.

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Thumann, J., Grill, E., Winnacker, E. L., & Zenk, M. H. (1991). Reactivation of metal-requiring apoenzymes by phytochelatin-metal complexes. FEBS Letters, 284(1), 66–69. https://doi.org/10.1016/0014-5793(91)80763-S

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