Receptor-regulated formation of GTP[γS] with subsequent persistent Gs-protein activation in membranes of human platelets

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Abstract

Preincubation of human platelet membranes with the ATP analog ATP[γS] led to persistent adenylate cyclase activation. This stimulation was increased by copreincubation with PGE1 and obliterated by removing endogenous GDP by the NTP-regenerating system, creatine phosphate plus creatine kinase. PGE1 partially reversed the action of the regenerating system. Control formation of GTP[γS] from ATP[γS] and GDP in platelet membranes was apparently not stimulated by PGE1. In contrast, in the presence of creatine phosphate plus creatine kinase, which prevented formation of GTP[γS], PGE1 stimulated formation of this GTP analog, by partially reversing the action of the NTP-regenerating system. The data indicate that GTP[γS] can be formed by a membrane-associated nucleoside diphosphokinase from ATP[γS] and GDP, resulting in persistent Gs-protein activation, and that this process can be stimulated by an agonist-activated receptor. © 1989.

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Wieland, T., & Jakobs, K. H. (1989). Receptor-regulated formation of GTP[γS] with subsequent persistent Gs-protein activation in membranes of human platelets. FEBS Letters, 245(1–2), 189–193. https://doi.org/10.1016/0014-5793(89)80219-4

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