Recognition and differentiation of various Penicillium species, Penicillium citrinum, Penicillium notatum, Penicillium oxalicum and Penicillium frequentans, using random amplified polymorphic DNA-polymerase chain reaction technique

  • Saeednejad Z
  • Sabokbar A
  • Bakhtiari A
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Abstract

Mol. methods have been established to make better sensitivity and specificity for differentiation of pathogenic and allergenic fungi. The purpose of this study was to obtain an appropriate mol. pattern for differentiation of Penicillium species understudy. In this study, 30 isolates of four Penicillium species including Penicillium citrinum, Penicillium notatum, Penicillium oxalicum and Penicillium frequentans were used. First, isolates were cultured. Then, for obtaining the fungal mats, fungi were grown into Sabouraud glucose broth. Freeze and Thaw and glass beads were used for cell disruption and DNA was extd. by phenol-chloroform method. RAPD-PCR technique was performed using three primers. The results showed that each primer produced a different reprodn. pattern. A total of 2 to 17 bands were obsd. in all the isolates of Penicillium, except P. citrinum C2 and P. frequentans F7. According to primer 1, mol. patterns of Penicillium species were approx. similar, but multiplied patterns were not completely similar. Having used primer 2, multiplied patterns in P. oxalicum and P. notatum were to some extent similar, but there were some obsd. differences between P. frequentans and two other species P. oxalicum and P. notatum. P. citrinum, P. oxalicum and P. notatum had approx. the same patterns using primer 3, but some differences were obsd. in P. frequentans with three other species. In conclusion, the interspecies differences were demonstrated with all the primers. Primers 1 and 2 were able, to some extent, to differentiate these species, while primers 1 and 3 sepd. P. frequentans from the others. [on SciFinder(R)]

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APA

Saeednejad, Z. L., Sabokbar, A., & Bakhtiari, A. (2016). Recognition and differentiation of various Penicillium species, Penicillium citrinum, Penicillium notatum, Penicillium oxalicum and Penicillium frequentans, using random amplified polymorphic DNA-polymerase chain reaction technique. African Journal of Microbiology Research, 6(39), 6793–6798. https://doi.org/10.5897/ajmr12.1115

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