The role of IL-7 and pre-TCR signaling during T cell development has been well characterized in murine but not in human system. We and others have reported that human BM hematopoietic progenitor cells (HPCs) display poor proliferation, inefficient double negative (DN) to double positive (DP) transition and no functional maturation in the in vitro OP9-Delta-like 1 (DL1) culture system.
Patel, E. S., Okada, S., Hachey, K., Yang, L. jun, Durum, S. K., Moreb, J. S., & Chang, L. J. (2012). Regulation of in vitro human T cell development through interleukin-7 deprivation and anti-CD3 stimulation. BMC Immunology, 13. https://doi.org/10.1186/1471-2172-13-46