Release test of alliin/alliinase double-layer tablet by HPLC - Allicin determination

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Abstract

A simple, precise and accurate method was developed and validated for the determination of allicin release from alliin/alliinase double-layer tablets. According to Appendix XC II of Chinese Pharmacopoeia 2010 edition Volume II, a small glass-method was adopted at the rotational speed of 100 r/min using 100 mL phosphate buffer (pH 6.8) as release medium. The release amount was determined by HPLC with a C18 column (250 mm×4.6 mm, 5 μm) using the mobile phase consisting of methanol -0.4% carboxylic acid (65:35) at a flow rate of 1 mL/min and UV detection at 242 nm. The current method demonstrates good linearity over the range 4.052-405.2 μg/mL (r2=0.9999) with an average recovery of 105.5%(RSD=1.25%). The accumulative release of alliin/alliinase double-layer tablets had good homogeneity for within- and between-batches. The method established is simple, accurate and repeatable for the determination of allicin release from alliin/alliinase double-layer tablets. © 2013 Xi'an Jiaotong University.

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APA

Liang, Y., Zhang, J. J., Zhang, Q. B., Wang, Z. X., Yin, Z. N., Li, X. X., … Ye, L. M. (2013). Release test of alliin/alliinase double-layer tablet by HPLC - Allicin determination. Journal of Pharmaceutical Analysis, 3(3), 187–192. https://doi.org/10.1016/j.jpha.2012.08.001

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