RNA fingerprinting of microbial community in the rhizosphere soil of grain legumes

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Microbial structural and expression profiles of the rhizospheres of three legumes, faba beans, peas and white lupin, were compared by RNA-arbitrarily primed PCR technique. Two different primers, M13 reverse and 10-mer primers, were used in the amplification and products resolved on non-denaturing polyacrylamide gel. With both DNA and RNA profiles Lupinus and Pisum rhizospheres were more similar to each other than to Vicia rhizosphere. The RAP-PCR products were also dot blotted and probed for bacterial peptidase transcripts. Plant-dependent rhizosphere effect was evident by the marked absence of transcripts for bacterial neutral metallopeptidase in Lupinus rhizosphere. The results of dot blot were further confirmed by RT-PCR for the expression of bacterial neutral metallopeptidase in the three rhizospheres. © 2004 Federation of European Microbiological Societies. Published by Elsevier B.V. All rights reserved.




Sharma, S., Aneja, M. K., Mayer, J., Schloter, M., & Munch, J. C. (2004). RNA fingerprinting of microbial community in the rhizosphere soil of grain legumes. FEMS Microbiology Letters, 240(2), 181–186. https://doi.org/10.1016/j.femsle.2004.09.026

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