RNase P-Mediated sequence-specific cleavage of RNA by engineered external guide sequences

4Citations
Citations of this article
19Readers
Mendeley users who have this article in their library.

Abstract

The RNA cleavage activity of RNase P can be employed to decrease the levels of specific RNAs and to study their function or even to eradicate pathogens. Two different technologies have been developed to use RNase P as a tool for RNA knockdown. In one of these, an external guide sequence, which mimics a tRNA precursor, a well-known natural RNase P substrate, is used to target an RNA molecule for cleavage by endogenous RNase P. Alternatively, a guide sequence can be attached to M1 RNA, the (catalytic) RNase P RNA subunit of Escherichia coli. The guide sequence is specific for an RNA target, which is subsequently cleaved by the bacterial M1 RNA moiety. These approaches are applicable in both bacteria and eukaryotes. In this review, we will discuss the two technologies in which RNase P is used to reduce RNA expression levels.

Cite

CITATION STYLE

APA

Derksen, M., Mertens, V., & Pruijn, G. J. M. (2015, November 9). RNase P-Mediated sequence-specific cleavage of RNA by engineered external guide sequences. Biomolecules. MDPI AG. https://doi.org/10.3390/biom5043029

Register to see more suggestions

Mendeley helps you to discover research relevant for your work.

Already have an account?

Save time finding and organizing research with Mendeley

Sign up for free