Role of the DELSEED loop in torque transmission of F1-ATPase

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Abstract

F1-ATPase is an ATP-driven rotary motor that generates torque at the interface between the catalytic β-subunits and the rotor γ-subunit. The β-subunit inwardly rotates the C-terminal domain upon nucleotide binding/dissociation; hence, the region of the C-terminal domain that is in direct contact with γ - termed the DELSEED loop - is thought to play a critical role in torque transmission. We substituted all the DELSEED loop residues with alanine to diminish specific DELSEED loop-γ interactions and with glycine to disrupt the loop structure. All the mutants rotated unidirectionally with kinetic parameters comparable to those of the wild-type F1, suggesting that the specific interactions between DELSEED loop and γ is not involved in cooperative interplays between the catalytic β-subunits. Glycine substitution mutants generated half the torque of the wild-type F1, whereas the alanine mutant generated comparable torque. Fluctuation analyses of the glycine/alanine mutants revealed that the γ-subunit was less tightly held in the α3β 3-stator ring of the glycine mutant than in the wild-type F 1 and the alanine mutant. Molecular dynamics simulation showed that the DELSEED loop was disordered by the glycine substitution, whereas it formed an α-helix in the alanine mutant. Our results emphasize the importance of loop rigidity for efficient torque transmissions. © 2012 Biophysical Society.

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Tanigawara, M., Tabata, K. V., Ito, Y., Ito, J., Watanabe, R., Ueno, H., … Noji, H. (2012). Role of the DELSEED loop in torque transmission of F1-ATPase. Biophysical Journal, 103(5), 970–978. https://doi.org/10.1016/j.bpj.2012.06.054

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