Role of the miR-17 92 cluster family in cerebellar and medulloblastoma development

  • Zindy F
  • Kawauchi D
  • Lee Y
  • et al.
Citations of this article
Mendeley users who have this article in their library.


© 2014, Company of Biologists Ltd. All rights reserved. The miR-17-92 cluster family is composed of three members encoding microRNAs that share seed sequences. To assess their role in cerebellar and medulloblastoma (MB) development, we deleted the miR-17-92 cluster family in Nestin-positive neural progenitors and in mice heterozygous for the Sonic Hedgehog (SHH) receptor Patched 1 (Ptch1 +/- ). We show that mice in which we conditionally deleted the miR-17-92 cluster (miR-17-92 floxed/floxed ; Nestin-Cre + ) alone or together with the complete loss of the miR-106b-25 cluster (miR-106b-25 -/- ) were born alive but with small brains and reduced cerebellar foliation. Remarkably, deletion of the miR-17-92 cluster abolished the development of SHH-MB in Ptch1 +/- mice. Using an orthotopic transplant approach, we showed that granule neuron precursors (GNPs) purified from the cerebella of postnatal day 7 (P7) Ptch1 +/- ; miR-106b-25 -/- mice and overexpressing Mycn induced MBs in the cortices of naïve recipient mice. In contrast, GNPs purified from the cerebella of P7 Ptch1 +/- ; miR-17-92 floxed/floxed ; Nestin-Cre + animals and overexpressing Mycn failed to induce tumors in recipient animals. Taken together, our findings demonstrate that the miR-17-92 cluster is dispensable for cerebellar development, but required for SHH-MB development.




Zindy, F., Kawauchi, D., Lee, Y., Ayrault, O., Ben Merzoug, L., McKinnon, P. J., … Roussel, M. F. (2014). Role of the miR-17 92 cluster family in cerebellar and medulloblastoma development. Biology Open, 3(7), 597–605.

Register to see more suggestions

Mendeley helps you to discover research relevant for your work.

Already have an account?

Save time finding and organizing research with Mendeley

Sign up for free