The role of short homology repeats and TdT in generation of the invariant γδ antigen receptor repertoire in the fetal thymus

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Abstract

Fetal thymic and adult epithelial Vγ3+ and Vγ4+ T cells express γδ antigen receptors (TCR) with invariant junctions lacking N nucleotides. Using transgenic recombination substrates, we show that di- or trinucleotide repeats, either in the coding region or in P elements, have strong effects on the site of recombination. In other mice bearing a terminal deoxynucleotidyl transferase (TdT) transgene under the control of the CD2 promoter, we found that the frequency of canonical junctions was markedly reduced with a concomitant increase in in-frame noncanonical junctions with N nucleotides. Together, our results show that short homology repeats direct the site of rearrangement and thus play a critical role in the generation of γδ T cell receptor canonical junctions. Increased TdT activity in Vγ3+ T cells has a inhibitory effect on junctional homogeneity in these cells. © 1995.

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Zhang, Y., Cado, D., Asarnow, D. M., Komori, T., Alt, F. W., Raulet, D. H., & Allison, J. P. (1995). The role of short homology repeats and TdT in generation of the invariant γδ antigen receptor repertoire in the fetal thymus. Immunity, 3(4), 439–447. https://doi.org/10.1016/1074-7613(95)90173-6

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