Schizosaccharomyces pombe UDP-galactose transporter: Identification of its functional form through cDNA cloning and expression in mammalian cells

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Abstract

The Schizosaccharomyces pombe UDP-galactose transporter cDNA (SpUGT cDNA), encoding the product of the gms1+ gene which consists of two exon sequences separated by a 173-bp intron, was cloned by RT-PCR. Its product, a hydrophobic protein of 353 amino acid residues resembling its human counterpart, was expressed in the Golgi membranes of UDP-galactose transporter-deficient Lec8 cells, and complemented the genetic defect of the mutant cells. This indicated that SpUGT cDNA encodes the functional S. pombe UDP-galactose transporter. The product of an ORF found in the second exon, which was previously assumed to be the S. pombe UDP-galactose transporter, thus represents an inactive, truncated form of the SpUGT protein. Copyright (C) 1999 Federation of European Biochemical Societies.

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Segawa, H., Ishida, N., Takegawa, K., & Kawakita, M. (1999). Schizosaccharomyces pombe UDP-galactose transporter: Identification of its functional form through cDNA cloning and expression in mammalian cells. FEBS Letters, 451(3), 295–298. https://doi.org/10.1016/S0014-5793(99)00596-7

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