Sda1, a Cys2-His2Zinc Finger Transcription Factor, Is Involved in Polyol Metabolism and Fumonisin B1Production in Fusarium verticillioides

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Abstract

The ubiquitous ascomycete Fusarium verticillioides causes ear rot and stalk rot of maize, both of which reduce grain quality and yield. Additionally, F. verticillioides produces the mycotoxin fumonisin B 1 (FB 1 ) during infection of maize kernels, and thus potentially compromises human and animal health. The current knowledge is fragmentary regarding the regulation of FB 1 biosynthesis, particularly when considering interplay with environmental factors such as nutrient availability. In this study, SDA1 of F. verticillioides, pre dicted to encode a Cys-2 His-2 zinc finger transcription factor, was shown to play a key role in catabolizing select carbon sources. Growth of the SDA1 knock-out mutant (Δsda1) was completely inhibited when sorbitol was the sole carbon source and was severely impaired when exclusively provided mannitol or glycerol. Deletion of SDA1 unexpectedly increased FB 1 biosynthesis, but reduced arabitol and mannitol biosynthesis, as compared to the wild-type progenitor. Trichoderma reesei ACE1, a regulator of cellulase and xylanase expression, complemented the F. verticillioides Δsda1 mutant, which indicates that Ace1 and Sda1 are functional orthologs. Taken together, the data indicate that Sda1 is a transcriptional regulator of carbon metabolism and toxin production in F. verticillioides. © 2013 Malapi-Wight et al.

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Malapi-Wight, M., Smith, J., Campbell, J., Bluhm, B. H., & Shim, W. B. (2013). Sda1, a Cys2-His2Zinc Finger Transcription Factor, Is Involved in Polyol Metabolism and Fumonisin B1Production in Fusarium verticillioides. PLoS ONE, 8(7). https://doi.org/10.1371/journal.pone.0067656

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