Selecting temperature for protein crystallization screens using the temperature dependence of the second virial coefficient

4Citations
Citations of this article
31Readers
Mendeley users who have this article in their library.

Abstract

Protein crystals usually grow at a preferable temperature which is however not known for a new protein. This paper reports a new approach for determination of favorable crystallization temperature, which can be adopted to facilitate the crystallization screening process. By taking advantage of the correlation between the temperature dependence of the second virial coefficient (B 22 ) and the solubility of protein, we measured the temperature dependence of B 22 to predict the temperature dependence of the solubility. Using information about solubility versus temperature, a preferred crystallization temperature can be proposed. If B 22 is a positive function of the temperature, a lower crystallization temperature is recommended; if B 22 shows opposite behavior with respect to the temperature, a higher crystallization temperature is preferred. Otherwise, any temperature in the tested range can be used. © 2011 Liu et al.

Cite

CITATION STYLE

APA

Liu, J., Yin, D. C., Guo, Y. Z., Wang, X. K., Xie, S. X., Lu, Q. Q., & Liu, Y. M. (2011). Selecting temperature for protein crystallization screens using the temperature dependence of the second virial coefficient. PLoS ONE, 6(3). https://doi.org/10.1371/journal.pone.0017950

Register to see more suggestions

Mendeley helps you to discover research relevant for your work.

Already have an account?

Save time finding and organizing research with Mendeley

Sign up for free