Sensitive determination of amlodipine besylate using bare/unmodified and DNA-modified screen-printed electrodes in tablets and biological fluids

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Abstract

The screen-printed technique is widely used as an efficient tool for electrochemical analysis in environment, clinical and agri-food areas. Significantly, it has the ability to transfer electrochemical laboratory experiments into the field. In the present work, we report a highly sensitive, simple, low-cost protocol for determination of amlodipine (AML) using bare/unmodified and DNA-modified screen-printed electrodes (SPEs). The immobilization of DNA molecules onto SPE offers promising robust and chemically stable molecular wires, which provides a unique opportunity for charge transfer processes. Consequently, the electroanalytical sensing of AML was explored at bare/unmodified and DNA-modified SPEs in a linear range between 0.066–1.0 μM and 0.066–2.0 μM with the detection limit (3σ) found to be 20.70 nM and 14.94 nM, whilst corresponding sensitivities of: 0.43 A L mol −1 and 4.23 A L mol −1 respectively. Although, the superior electrochemical signature of bare SPEs is evident, the immobilization of DNA onto SPEs enhances the sensitivity 10-times more than the bare SPEs. Furthermore, the optimized electroanalytical protocol using the unmodified SPEs, which requires no pre-treatment and electrode modification step, was then further applied to the determination of AML in real samples.

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Khairy, M., Khorshed, A. A., Rashwan, F. A., Salah, G. A., Abdel-Wadood, H. M., & Banks, C. E. (2017). Sensitive determination of amlodipine besylate using bare/unmodified and DNA-modified screen-printed electrodes in tablets and biological fluids. Sensors and Actuators, B: Chemical, 239, 768–775. https://doi.org/10.1016/j.snb.2016.07.165

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